Composite

Part:BBa_K4594021:Design

Designed by: Yueying Wang   Group: iGEM23_ShanghaiTech-China   (2023-10-04)


NSIII-2-PkaiBC-LuxA-LuxB-PpsbAI-LuxCDE-LuxG-LuxF-KanR-NSIII-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5851
    Illegal PstI site found at 641
    Illegal PstI site found at 8848
    Illegal PstI site found at 10205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 641
    Illegal PstI site found at 8848
    Illegal PstI site found at 10205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7091
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5851
    Illegal PstI site found at 641
    Illegal PstI site found at 8848
    Illegal PstI site found at 10205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5851
    Illegal PstI site found at 641
    Illegal PstI site found at 8848
    Illegal PstI site found at 10205
    Illegal AgeI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5209
    Illegal SapI site found at 2422


Design Notes

111


Source

11

References